6M6V

Crystal structure the toxin-antitoxin MntA-HepT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97982
Spacegroup name	P 21 21 2
Unit cell lengths	56.560, 225.700, 52.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.850 - 3.080
R-factor	0.2231
Rwork	0.221
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yep
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.850	3.160
High resolution limit [Å]	3.080	3.080
Number of reflections	13153	4214
<I/σ(I)>	10.2
Completeness [%]	99.3
Redundancy	12.5
CC(1/2)	0.997	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	10 % (w/v) PEG 3350, 0.1M Magnesium acetate, 0.1M HEPS pH 7.0