6M6R
Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 8-mer ssRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2819 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 56.843, 56.843, 131.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.036 - 1.890 |
R-factor | 0.1797 |
Rwork | 0.178 |
R-free | 0.22130 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.991 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.040 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.142 | 1.715 |
Rmeas | 0.158 | 1.950 |
Number of reflections | 17289 | 984 |
<I/σ(I)> | 12 | 1.7 |
Completeness [%] | 96.3 | 88.4 |
Redundancy | 9.2 | 7.5 |
CC(1/2) | 0.996 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M Tris pH 8.5, 25% w/v polyethylene glycol 3350 |