6M5Z
Catalytic domain of GH30 xylanase C from Talaromyces cellulolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.559, 52.305, 98.535 |
| Unit cell angles | 79.74, 88.60, 84.30 |
Refinement procedure
| Resolution | 49.010 - 1.650 |
| Rwork | 0.148 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iuj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS (0.82) |
| Data scaling software | XDS (0.82) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.073 | 0.633 |
| Rmeas | 0.087 | 0.748 |
| Rpim | 0.046 | 0.395 |
| Number of reflections | 87809 | 8491 |
| <I/σ(I)> | 11.8 | 2 |
| Completeness [%] | 96.8 | 93.5 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.998 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 14% PEG 8000, 100 mM MES, 200 mM calcium acetate |
