6M5J
U shaped head to head four-way junction in d(TTCTGCTGCTGAA/TTCTGCAGCTGAA) sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-26 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.630, 62.086, 30.747 |
| Unit cell angles | 90.00, 121.84, 90.00 |
Refinement procedure
| Resolution | 19.221 - 1.650 |
| R-factor | 0.1876 |
| Rwork | 0.187 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m4t |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 3.530 | 1.640 |
| Rmerge | 0.045 | 0.029 | 0.230 |
| Rmeas | 0.053 | 0.034 | 0.279 |
| Rpim | 0.028 | 0.018 | 0.156 |
| Total number of observations | 37937 | ||
| Number of reflections | 11117 | 1126 | 1038 |
| <I/σ(I)> | 21.8 | ||
| Completeness [%] | 97.8 | 95.8 | 91.9 |
| Redundancy | 3.4 | 3.5 | 2.8 |
| CC(1/2) | 0.998 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | MES (pH=6.5) 50 mM, KCl 200 mM, MPD 35%, CoCl2 10 mM |
