6M5I
Crystal structure of 2019-nCoV nsp7-nsp8c complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.944, 69.376, 101.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.062 - 2.496 |
R-factor | 0.216 |
Rwork | 0.208 |
R-free | 0.29170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f22 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.496 | 2.500 |
Number of reflections | 6534 | 892 |
<I/σ(I)> | 8.01 | |
Completeness [%] | 99.7 | |
Redundancy | 6.85 | |
CC(1/2) | 0.998 | 0.093 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Magnesium formate dihydrate,15% PEG3350. |