6M4T
U shaped head to head four-way junction in d(TTCTGCTGCTGAA) sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.91932 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.204, 62.631, 29.743 |
Unit cell angles | 90.00, 121.29, 90.00 |
Refinement procedure
Resolution | 19.734 - 1.550 |
R-factor | 0.1675 |
Rwork | 0.165 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.610 |
High resolution limit [Å] | 1.543 | 3.340 | 1.550 |
Rmerge | 0.042 | 0.025 | 0.604 |
Rmeas | 0.050 | 0.029 | 0.714 |
Rpim | 0.026 | 0.015 | 0.377 |
Number of reflections | 13080 | 1321 | 1250 |
<I/σ(I)> | 16.1 | ||
Completeness [%] | 99.2 | 98.4 | 95.3 |
Redundancy | 3.7 | 3.6 | 3.4 |
CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | MES (pH=6.5) 50mM, MPD 35 %, Potassium chloride 200mM, CoCl2 20mM |