6M3Y
Crystal structure of pilus adhesin, SpaC from Lactobacillus rhamnosus GG - open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.045, 110.023, 128.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.530 - 1.930 |
R-factor | 0.2086 |
Rwork | 0.207 |
R-free | 0.24757 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.311 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.531 | 2.042 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.055 | 0.575 |
Rpim | 0.029 | 0.352 |
Number of reflections | 52205 | 2611 |
<I/σ(I)> | 14.3 | 1.9 |
Completeness [%] | 83.8 | |
Redundancy | 4 | 2.8 |
CC(1/2) | 0.999 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 295 | 0.1 M sodium sulphate, 0.1 M Bis-Tris propane pH 6.5, 25% (w/v) PEG 3350 |