6M3M

Crystal structure of SARS-CoV-2 nucleocapsid protein N-terminal RNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-28
Detector	OXFORD RUBY CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.888, 92.685, 97.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.920 - 2.700
Rwork	0.258
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2og3
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.920	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.104	0.317
Rmeas	0.114	0.344
Rpim	0.044	0.130
Number of reflections	15133	1481
<I/σ(I)>	14.97	2.9
Completeness [%]	99.5	99.8
Redundancy	6.5	6.8
CC(1/2)	0.990	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	20 mM sodium acetate, 100 mM sodium cacodylate (pH 6.5), 26 % PEG 8000