6M3G
Crystal structure of human HPF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-17 |
Detector | Nonius Kappa CCD |
Wavelength(s) | 0.97853 |
Spacegroup name | H 3 2 |
Unit cell lengths | 89.981, 89.981, 226.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.860 - 1.570 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.20480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.942 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rpim | 0.017 | |
Number of reflections | 49462 | 2567 |
<I/σ(I)> | 45.2 | |
Completeness [%] | 99.3 | |
Redundancy | 9.6 | |
CC(1/2) | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 300 | 0.1 M Tris-pH 8.1, 0.2 M potassium acetate, 20% w/v Polyethylene glycol (PEG) 3350 |