6M3C

hAPC-h1573 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	124.339, 124.339, 666.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.385 - 3.700
R-factor	0.2796
Rwork	0.278
R-free	0.30220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aut
RMSD bond length	0.004
RMSD bond angle	1.101
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.790
High resolution limit [Å]	3.700	3.700
Rpim	0.149
Number of reflections	56425	3756
<I/σ(I)>	6.14
Completeness [%]	98.4
Redundancy	5.5
CC(1/2)		0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M sodium fluoride, 20% (w/v) PEG 3350