6M37

The crystal structure of B. subtilis RsbV/RsbW complex in the hexagonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97933
Spacegroup name	P 64 2 2
Unit cell lengths	158.200, 158.200, 96.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.668 - 3.100
R-factor	0.2232
Rwork	0.222
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1thn 1til
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.090	0.654
Number of reflections	13426	2377
<I/σ(I)>	15.3	3.6
Completeness [%]	99.9
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	20% w/v PEG3350 and 200 mM Potassium formate, ADP