6M34
Structural and functional insights into an Archaeal synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-25 |
Detector | DECTRIS PILATUS3 R 1M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.500, 79.750, 70.300 |
Unit cell angles | 90.00, 111.87, 90.00 |
Refinement procedure
Resolution | 39.910 - 2.900 |
R-factor | 0.248 |
Rwork | 0.247 |
R-free | 0.26900 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.910 | 39.910 | 3.070 |
High resolution limit [Å] | 2.890 | 8.530 | 2.890 |
Rmerge | 0.164 | 0.062 | 0.714 |
Rmeas | 0.178 | 0.067 | 0.770 |
Total number of observations | 225465 | ||
Number of reflections | 16504 | 1268 | 5228 |
<I/σ(I)> | 9.65 | 26.92 | 2.93 |
Completeness [%] | 99.0 | 98.8 | 99.1 |
Redundancy | 7.025 | 7.245 | 7.204 |
CC(1/2) | 0.995 | 0.997 | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293.15 | 200 mM NaCl, 20% (w/v) PEG400, and 200 mM Tris-HCl (pH 7.0). |