6M33
Crystal structure of Homo Sapian GCP6 N-terminus and Mozart1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-28 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.99984 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.466, 69.466, 118.186 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.771 - 3.294 |
R-factor | 0.229930059088 |
Rwork | 0.222 |
R-free | 0.29826 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.410 |
High resolution limit [Å] | 3.290 | 3.290 |
Number of reflections | 5211 | 505 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 98.2 | |
Redundancy | 3.1 | |
CC(1/2) | 0.997 | 0.695 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5M Ammonium chloride, 0.1M Bis-Tris propane p.H 7.0 |