6M2K
Uncommon structural features of rabbit MHC class I (RLA-A1) complexed with rabbit haemorrhagic disease virus (RHDV) derived peptide, VP60-10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2019-10-15 |
Detector | SDMS |
Wavelength(s) | 1.03923 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.612, 74.303, 152.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 2.590 |
R-factor | 0.219 |
Rwork | 0.215 |
R-free | 0.29380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5deg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.018 |
Data reduction software | MOSFLM |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.112 | 0.758 |
Number of reflections | 18060 | 18060 |
<I/σ(I)> | 2.78 | |
Completeness [%] | 99.9 | |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BIS-TRIS PH 6.5, 20% w/v PEG 5000 |