6M24

Uncommon structural features of rabbit MHC class I (RLA-A1) complexed with rabbit haemorrhagic disease virus (RHDV) derived peptide, VP60-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-07-10
Detector	SDMS
Wavelength(s)	1.03853
Spacegroup name	P 21 21 21
Unit cell lengths	49.578, 76.218, 155.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.390 - 2.290
R-factor	0.1968
Rwork	0.194
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5deg
RMSD bond length	0.008
RMSD bond angle	0.893
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.200	2.300
Rmerge	0.101	0.857
Number of reflections	27014	27014
<I/σ(I)>	2.75
Completeness [%]	99.9
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.2M magnesium chloride hexahydrate, 0.1M sodium citrate tribasic, pH 5.0, 10% w/v polyethylene glycol 20000