6M20

Crystal structure of Plasmodium falciparum hexose transporter PfHT1 bound with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	120.000, 68.300, 175.300
Unit cell angles	90.00, 103.30, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.2457
Rwork	0.245
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zw9
RMSD bond length	0.004
RMSD bond angle	0.614
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	84480	8948
<I/σ(I)>	7.89
Completeness [%]	98.8
Redundancy	3.43
CC(1/2)	0.998	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	291	0.1 M NaCl, 0.1 M MES pH 5.7, 26% (w/v) PEG 400 and 2% (w/v) Benzamidine hydrochloride