6M0E
Beijerinckia indica beta-fructosyltransferase complexed with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.377, 81.521, 99.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.350 |
| Rwork | 0.150 |
| R-free | 0.16630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m0d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.470 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.047 | 0.468 |
| Rpim | 0.020 | 0.190 |
| Number of reflections | 96105 | 13691 |
| <I/σ(I)> | 19.4 | 4.1 |
| Completeness [%] | 98.9 | 97.7 |
| Redundancy | 6.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30% (w/v) polyethylene glycol 10000, 0.2 M MgCl2, 0.1 M Na K tartrate, 0.1 M Tris-HCl |
