6M0D
Beijerinckia indica beta-fructosyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.891, 80.900, 92.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.300 - 2.200 |
Rwork | 0.222 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w18 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.636 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.300 | 2.230 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.125 | 0.338 |
Rpim | 0.054 | 0.140 |
Number of reflections | 20772 | 1750 |
<I/σ(I)> | 9.6 | 4.8 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.4 | |
CC(1/2) | 0.992 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30% (w/v) polyethylene glycol 10000, 0.2 M MgCl2, 0.1 M Na K tartrate, 0.1 M Tris-HCl |