6M01
The structure of HitB-HitD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-02 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.090, 91.680, 73.740 |
| Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
| Resolution | 45.880 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jjq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.566 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.569 |
| Number of reflections | 38673 | 5619 |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris-HCl, sodium acetate, PEG 4000, PEG 400 |
