6LZM
COMPARISON OF THE CRYSTAL STRUCTURE OF BACTERIOPHAGE T4 LYSOZYME AT LOW, MEDIUM, AND HIGH IONIC STRENGTHS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | rotating-anode X-ray tube |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 298 |
| Detector technology | photographic film |
| Detector | OSCILLATION CAMERA |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.000, 61.000, 96.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.16 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.090 |
| Data scaling software | AGROVATA / ROTAVATA |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.059 * |
| Total number of observations | 38400 * |
| Number of reflections | 14409 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | T4 lysozyme | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | 0.4 (M) | ||
| 4 | 1 | reservoir | PEG3500 | 30 (%) | |
| 5 | 1 | reservoir | 2-mercaptoethanol | 14 (mM) |
