6LYS
Structure of the BAM complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 117.073, 117.073, 429.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.050 |
R-factor | 0.2448 |
Rwork | 0.243 |
R-free | 0.28440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d0o |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.050 | 3.050 |
Number of reflections | 53217 | 3492 |
<I/σ(I)> | 20.7 | |
Completeness [%] | 99.9 | |
Redundancy | 12.8 | |
CC(1/2) | 0.998 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 27-30% PEG 400, 100mM NaCl |