6LYR
Structure of the BAM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.877, 116.877, 430.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.370 - 3.280 |
| R-factor | 0.2482 |
| Rwork | 0.246 |
| R-free | 0.28020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d0o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Number of reflections | 44370 | 3160 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 18.7 | |
| CC(1/2) | 1.000 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 27-30% PEG 400, 100mM NaCl |
