6LYQ

Structure of the BAM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	117.274, 117.274, 429.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.410 - 3.190
R-factor	0.2665
Rwork	0.265
R-free	0.29320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0o
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.190	3.190
Number of reflections	47870	3227
<I/σ(I)>	21.6
Completeness [%]	99.4
Redundancy	21.6
CC(1/2)	1.000	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	27-30% PEG 400, 100mM NaCl