6LYE

Crystal Structure of mimivirus UNG Y322F in complex with UGI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97933
Spacegroup name	P 61
Unit cell lengths	104.230, 104.230, 83.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.133 - 3.100
R-factor	0.1868
Rwork	0.173
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x55
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.133	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.127	0.604
Number of reflections	9163	1685
<I/σ(I)>	8.3
Completeness [%]	97.6
Redundancy	4.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	16.75% (v/v) PEG3350, 8% (v/v) PEG400, and 0.1 M sodium acetate/acetic acid pH 5.5