6LYD

Crystal Structure of mimivirus UNG Y322L in complex with UGI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97933
Spacegroup name	P 61
Unit cell lengths	103.766, 103.766, 81.523
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.053 - 2.598
R-factor	0.1756
Rwork	0.170
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x55
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.598	2.600
Rmerge	0.091	0.458
Number of reflections	15494	770
<I/σ(I)>	30.6
Completeness [%]	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	10% PEG 8000 (v/v), 40 mM potassium phosphate monobasic, 20% glycerol (v/v), and 0.2 uL of 5% ethyl acetate (v/v)