6LYA
PylRS C-terminus domain mutant bound with 1-Methyl-L-tryptophan and AMPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2017-11-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 |
Unit cell lengths | 105.450, 105.450, 71.824 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.502 - 1.591 |
R-factor | 0.178800562783 |
Rwork | 0.178 |
R-free | 0.19419 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zce |
RMSD bond length | 0.013 |
RMSD bond angle | 1.192 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.680 | 1.650 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmeas | 0.073 | 1.232 |
Rpim | 0.025 | 0.410 |
Number of reflections | 60796 | 5945 |
<I/σ(I)> | 15.5 | |
Completeness [%] | 99.8 | |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 10% PEG 8000, 10% Ethylene Glycerol |