6LXU
Crystal structure of methionine gamma-lyase from Fusobacterium nucleatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.947, 99.915, 138.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.780 - 1.190 |
| R-factor | 0.15243 |
| Rwork | 0.152 |
| R-free | 0.16101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rfv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.419 |
| Data reduction software | DIALS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.060 | 1.220 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.060 | 0.250 |
| Rmeas | 0.064 | 0.285 |
| Rpim | 0.017 | 0.097 |
| Number of reflections | 145156 | 10334 |
| <I/σ(I)> | 26.3 | 6.7 |
| Completeness [%] | 99.7 | 96.8 |
| Redundancy | 13.5 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 41% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.2M Ammonium nitrate |
