6LXS
CWA protein-SdrC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-30 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.485, 76.837, 79.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.290 - 1.580 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.069 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.290 | 1.623 |
| High resolution limit [Å] | 1.580 | 1.582 |
| Rmerge | 0.104 | |
| Number of reflections | 41307 | 2822 |
| <I/σ(I)> | 39.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.8 | |
| CC(1/2) | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 0.2M Calcium acetate hydrate, 0.1M Sodium cacodylate trihydrate pH 6.5, 18% Polyethylene glycol 8000 |
