6LXR
TvCyP2 in apo form 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.079, 54.539, 59.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.170 - 2.560 |
| R-factor | 0.1741 |
| Rwork | 0.168 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yba |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.170 | 2.653 |
| High resolution limit [Å] | 2.560 | 2.561 |
| Number of reflections | 5340 | 293 |
| <I/σ(I)> | 18.5 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.978 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 1.0 M Sodium citrate, 0.2 M Sodium chloride, 0.1 M Tris pH 7.0 |
