6LXP
TvCyP2 in apo form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.823, 54.262, 59.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.130 - 2.350 |
| R-factor | 0.1629 |
| Rwork | 0.155 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yba |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.130 | 2.441 |
| High resolution limit [Å] | 2.350 | 2.356 |
| Number of reflections | 6685 | 487 |
| <I/σ(I)> | 16.8 | 2.8 |
| Completeness [%] | 91.4 | 91.8 |
| Redundancy | 4.5 | |
| CC(1/2) | 0.970 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 288 | 0.1 M TRIS pH 8.5, 0.3 M Magnesium formate dihydrate |
