6LXO

TvCyP2 in apo form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.975
Spacegroup name	P 21 21 21
Unit cell lengths	50.811, 56.189, 59.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.090 - 1.890
R-factor	0.1433
Rwork	0.139
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yba
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.090	1.959
High resolution limit [Å]	1.890	1.891
Number of reflections	14047	1363
<I/σ(I)>	33.813	10.6
Completeness [%]	98.2	90.9
Redundancy	4.7
CC(1/2)	0.993	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	288	0.1 M Citric Acid pH 5.0, 1.6 M Ammonium Sulfate