6LXN

Crystal structure of C-terminal DNA-binding domain of Escherichia coli OmpR in complex with F1-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-18
Detector	RAYONIX MX300-HS
Wavelength(s)	0.975
Spacegroup name	P 63
Unit cell lengths	70.753, 70.753, 165.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.730 - 2.930
R-factor	0.178
Rwork	0.170
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lxl
RMSD bond length	0.009
RMSD bond angle	1.127
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.730	3.040
High resolution limit [Å]	2.930	2.930
Number of reflections	8766	985
<I/σ(I)>	45.5
Completeness [%]	99.5
Redundancy	9.4
CC(1/2)	0.998	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1 M Magnesium acetate tetrahydrate, 0.05 M MED monohydrate, pH 5.6, 20% v/v (+/-)-2-Methyl-2,4,pentanediol