6LXL
Crystal structure of C-terminal DNA-binding domain of Escherichia coli OmpR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.975 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 60.297, 60.297, 83.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 3.560 |
R-factor | 0.2422 |
Rwork | 0.234 |
R-free | 0.31870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nhj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.542 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.880 | 3.690 |
High resolution limit [Å] | 3.560 | 3.560 |
Rmerge | 0.175 | |
Number of reflections | 2548 | 2039 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 100.0 | |
Redundancy | 8.4 | |
CC(1/2) | 0.965 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M CHES pH 9.5 10% PEG 3000 |