6LXL

Crystal structure of C-terminal DNA-binding domain of Escherichia coli OmpR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.975
Spacegroup name	P 43 21 2
Unit cell lengths	60.297, 60.297, 83.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 3.560
R-factor	0.2422
Rwork	0.234
R-free	0.31870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nhj
RMSD bond length	0.003
RMSD bond angle	0.542
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	3.690
High resolution limit [Å]	3.560	3.560
Rmerge	0.175
Number of reflections	2548	2039
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	8.4
CC(1/2)	0.965	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1 M CHES pH 9.5 10% PEG 3000