6LXK

Crystal structure of Z2B3 D102R Fab in complex with influenza virus neuraminidase from A/Serbia/NS-601/2014 (H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-05
Detector	Nonius Kappa CCD
Wavelength(s)	0.97930
Spacegroup name	P 32 2 1
Unit cell lengths	214.442, 214.442, 168.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.459 - 3.608
R-factor	0.2164
Rwork	0.215
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3beq 5w0d
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	51355	51355
<I/σ(I)>	6.4
Completeness [%]	100.0
Redundancy	8.3
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.1M magnesium acetate, 0.1M MES pH 6.5, 10% (w/v) PEG 10000