6LXH
Staphylococcus aureus surface protein-sdrc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-30 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.753, 120.333, 130.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.420 - 2.070 |
R-factor | 0.1729 |
Rwork | 0.170 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.812 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.420 | 2.123 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 59835 | 4110 |
<I/σ(I)> | 88.42 | |
Completeness [%] | 100.0 | |
Redundancy | 13.3 | |
CC(1/2) | 0.956 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 289 | 0.1M HEPES, 30% Jeffamine ED-2001, pH 7.0 |