6LX2
Potato D-enzyme complexed with CA26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.841, 120.413, 174.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.050 |
| R-factor | 0.16786 |
| Rwork | 0.166 |
| R-free | 0.19636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.776 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.062 | 0.347 |
| Number of reflections | 46979 | 6745 |
| <I/σ(I)> | 5.7 | 4.8 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 293 | 12% (w/v) PEG 8000, 100 mM HEPES, pH 7.6, 100 mM CaCl2 |
