6LX0

Structure of Leptospira santarosai serovar shermani LRR protein LSS11580

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-14
Detector	MAR CCD 130 mm
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	47.301, 47.301, 120.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.959 - 1.990
R-factor	0.199
Rwork	0.197
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pq8
RMSD bond length	0.007
RMSD bond angle	0.901
Data reduction software	ADDREF
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.030	2.020
High resolution limit [Å]	1.990	1.990
Number of reflections	10011	486
<I/σ(I)>	3.4
Completeness [%]	99.7	100
Redundancy	12.6	13.2
CC(1/2)	0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1M Tris hydrochloride (pH 8.5), 0.2M Sodium acetate trihydrate, 30% (w/v) Polyethylene glycol 4000