6LW4

Structure of Lpg2148/Lpg2149 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	67.524, 67.524, 205.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.230 - 2.700
R-factor	0.2456
Rwork	0.243
R-free	0.29850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5suj 5dpo
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.798
High resolution limit [Å]	2.700	2.700
Rmerge	0.106	0.780
Number of reflections	13640	623
<I/σ(I)>	31.3
Completeness [%]	99.8
Redundancy	18.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG3350, Magnesium chloride hexahydrate