6LW3

Crystal structure of RuvC from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	60.379, 90.119, 61.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.110 - 2.380
R-factor	0.1882
Rwork	0.185
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hjr
RMSD bond length	0.007
RMSD bond angle	0.828
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (1.13_2998)
Phasing software	PHENIX (v1.13)
Refinement software	PHENIX (v1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	26012	1272
<I/σ(I)>	28.4
Completeness [%]	99.6
Redundancy	6.8
CC(1/2)	0.995	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	0.2 M ammonium citrate tribasic pH 7.0, 20% (w/v) polyethylene glycol 3350