6LVW

Polyextremophilic Beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	P 63
Unit cell lengths	100.130, 100.130, 137.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.357 - 2.493
R-factor	0.2489
Rwork	0.246
R-free	0.30750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kwg
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	BALBES
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.070	50.070	2.560
High resolution limit [Å]	2.490	11.150	2.490
Rmerge	0.149	0.050	0.046
Rmeas	0.153	0.052	4.729
Rpim	0.034	0.012	1.162
Total number of observations	539186	5202	29793
Number of reflections	27127	320	1878
<I/σ(I)>	15	58	0.6
Completeness [%]	99.3	99	93.2
Redundancy	19.9	16.3	15.9
CC(1/2)	0.999	0.999	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	85 mM Tris-HCl (pH 8.5), 142 mM MgCl2, 2.78 M 1,6-hexanediol, 450 mM NDSB-195