6LVU

Crystal structure of apo acyl carrier protein from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	23.834, 61.955, 95.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.367 - 2.294
R-factor	0.228
Rwork	0.222
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ehs
RMSD bond length	0.008
RMSD bond angle	1.086
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	2.380
High resolution limit [Å]	2.294	4.950	2.300
Rmerge	0.081	0.054	0.201
Rmeas	0.088	0.058	0.218
Rpim	0.032	0.022	0.082
Number of reflections	6764	708	685
<I/σ(I)>	22.8
Completeness [%]	99.0	92.4	99.7
Redundancy	7.3	6.1	7.1
CC(1/2)		0.998	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	293	0.2M zinc acetate dihydrate, 20% PEG 3350