6LUT

Crystal structure of Serine Racemase from Dictyostelium discoideum.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.980
Spacegroup name	P 1
Unit cell lengths	40.642, 58.069, 64.530
Unit cell angles	87.40, 72.75, 69.60

Refinement procedure

Resolution	36.750 - 1.350
R-factor	0.2488
Rwork	0.248
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v71
RMSD bond length	0.007
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.496	36.754	1.420
High resolution limit [Å]	1.350	4.270	1.350
Rmerge		0.037	0.351
Rmeas	0.066	0.049	0.476
Rpim	0.044	0.032	0.319
Total number of observations	237696	6988	33241
Number of reflections	109316	3282	15195
<I/σ(I)>	8.7	19.3	2
Completeness [%]	94.4	89.6	90.1
Redundancy	2.2	2.1	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	PEG 1540, sodium chloride, MPD, PIPES