6LUP
Crystal structure of shark MHC CLASS I for 2.3 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-16 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 64 |
Unit cell lengths | 125.859, 125.859, 132.473 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.622 - 2.302 |
Rwork | 0.255 |
R-free | 0.28350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y91 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.362 |
High resolution limit [Å] | 2.300 | 2.302 |
Rmerge | 0.110 | 0.571 |
Number of reflections | 52646 | 3679 |
<I/σ(I)> | 21.185 | 4.267 |
Completeness [%] | 99.8 | 99.38 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | pH 7.5, 0.09 M HEPES sodium, 1.26 M Sodium citrate tribasic dihydrate,10% v/v Glycerol |