6LUJ
Crystal structure of the SAMD1 SAM domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2019-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.336, 69.336, 181.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.847 - 1.120 |
| R-factor | 0.1749 |
| Rwork | 0.175 |
| R-free | 0.17840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A model solved by Se-Met labelled sample |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.071 | 0.560 |
| Number of reflections | 190708 | 7808 |
| <I/σ(I)> | 49.7 | 2.3 |
| Completeness [%] | 97.9 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 2.1M ammonium sulfatesulphate, 0.2M magnesium chloride hexahydrate |
