6LUI
Crystal structure of the SAMD1 WH domain and DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97855 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.925, 45.925, 132.638 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.469 - 1.781 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24180 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.091 | 0.797 |
| Number of reflections | 16308 | 794 |
| <I/σ(I)> | 60 | 3.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 18.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.2M Calcium calcium acetate hydrate, 20% w/v Polyethylene polyethylene glycol 3350 |
