6LUH
High resolution structure of N(omega)-hydroxy-L-arginine hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 46.326, 47.057, 59.261 |
Unit cell angles | 83.57, 84.63, 70.13 |
Refinement procedure
Resolution | 44.090 - 1.500 |
R-factor | 0.2012 |
Rwork | 0.200 |
R-free | 0.23020 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6lug |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | SHARP (2.8) |
Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.090 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.108 | 0.449 |
Rmeas | 0.117 | 0.484 |
Rpim | 0.043 | 0.178 |
Total number of observations | 519387 | |
Number of reflections | 72157 | 3541 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 96.5 | 94.3 |
Redundancy | 7.2 | 7.4 |
CC(1/2) | 0.993 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 298 | PEG 4000, magnesium chloride |