6LUH

High resolution structure of N(omega)-hydroxy-L-arginine hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	46.326, 47.057, 59.261
Unit cell angles	83.57, 84.63, 70.13

Refinement procedure

Resolution	44.090 - 1.500
R-factor	0.2012
Rwork	0.200
R-free	0.23020
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6lug
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	SHARP (2.8)
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.090	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.108	0.449
Rmeas	0.117	0.484
Rpim	0.043	0.178
Total number of observations	519387
Number of reflections	72157	3541
<I/σ(I)>	11.2
Completeness [%]	96.5	94.3
Redundancy	7.2	7.4
CC(1/2)	0.993	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	298	PEG 4000, magnesium chloride