6LUG
Crystal structure of N(omega)-hydroxy-L-arginine hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.8924 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.440, 47.020, 59.350 |
| Unit cell angles | 83.44, 84.59, 70.31 |
Refinement procedure
| Resolution | 44.100 - 1.900 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.24960 |
| Structure solution method | SAD |
| Data reduction software | XDS (20190315) |
| Data scaling software | XSCALE (20190315) |
| Phasing software | SHARP (2.8) |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.106 | 0.288 |
| Rmeas | 0.110 | 0.318 |
| Rpim | 0.030 | 0.131 |
| Total number of observations | 434720 | |
| Number of reflections | 35027 | 1975 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 95.3 | 79.2 |
| Redundancy | 12.4 | 5.8 |
| CC(1/2) | 0.995 | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 298 | PEG 4000, Magnesium chloride |
