6LUF

Trans-acting mutant Y290A of the central AAA+ domain of the flagellar regulatory protein FlrC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-08-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	80.927, 154.127, 194.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.277 - 3.450
R-factor	0.1901
Rwork	0.188
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qhs
RMSD bond length	0.003
RMSD bond angle	0.918
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	3.640
High resolution limit [Å]	3.450	3.450
Number of reflections	32784	4707
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.990	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5% (w/v) PEG6000