6LUA

Cis-mutant R349A of the central AAA+ domain of the flagellar regulatory protein FlrC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	80.753, 154.633, 194.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 3.100
R-factor	0.193
Rwork	0.190
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qhs
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.163
Rpim	0.076
Number of reflections	45041	4337
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	5.5
CC(1/2)	0.992	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% (w/v) PEG6000, in 0.1M HEPES (pH 7.0) and 3% Glycerol