6LTY
DNA bound antitoxin HigA3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.615, 101.812, 58.027 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 41.790 - 3.280 |
R-factor | 0.2799 |
Rwork | 0.277 |
R-free | 0.32560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ltz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.277 | 3.277 |
Rmeas | 0.072 | 0.654 |
Number of reflections | 6656 | 316 |
<I/σ(I)> | 27.3 | |
Completeness [%] | 99.3 | |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 23% w/v PEG3350, 0.2 M sodium acetate trihydrate pH 7.0 |